Resources

Introduction to Developing DNA Reference Barcode Sequences

This how-to guide was developed collaboratively among West Coast OBON members including SIO, NOAA, LA Natural History Museum, and others. It emphasizes the importance of taxonomic expertise, sample preservation and curation, and sequence data quality.

Better Biomolecular Ocean Practices (BeBOP-OBON)

This resource has repositories that include: Minimum Information about an Omics Protocol (MIOP) specifications, biomolecular protocol templates, protocol examples, and digital assets to connect BeBOP to ODIS & Ocean InfoHub.

CalCOFI eDNA Protocols Repository

BeBOP-formatted protocols for collecting and processing eDNA samples on CalCOFI surveys. Under development, check back soon for more protocols!

NOAA ’omics data management guide

This guide was created to help ’omics users decide which data goes to which repositories (NCEI, NCBI, etc.), and how to deposit their data so that it will be FAIR (findable, accessible, interoperable, and reusable), including following community standards for raw data, processed data, and contextual metadata and environmental data.

A WC-OBON endorsed molecular methods guide

This guide includes information to help decision making for marker gene amplification and sequencing. This includes all protocols from member institutions of WC-OBON.

rCRUX: A rapid and versatile tool for generating reference libraries in R

This tool can generate comprehensive metabarcoding reference libraries for any bespoke locus.